Differential DNA methylation of PNR vs PS and MPAL. (A) Heatmap of methylation showing the top 500 differentially methylated loci in PNR vs PS. Scale depicts normalized methylation values at the probe loci. (B) GSEA of PNR vs PS leukemias shows increased methylation at targets of PRC2 in ESCs. (C) Differential methylation analysis strategy of a publicly available data set of MPAL vs single-phenotype acute leukemias³⁶ (D) Pathways enriched in MPAL-DMRs (n = 2204) detected by a hypergeometric Fisher exact test. (E) Venn diagram showing that the 70 overlapping regions between MPAL-DMRs and PNR-DMRs are enriched for SUZ12, JARID2, EZH2, and EED targets based on ENRICHR analysis.