Figure 5.
Transitory-Tex-CNI contributed to the development of cGVHD. (A-F) The first allo-HCT was performed as in Figure 1A, and recipient mice were orally administered with CSP or vehicle from days 0 to 14 after allo-HCT. A total of 1 × 106 T cells purified from vehicle- or CSP-treated recipients 14 days were adoptively transferred into lethally irradiated secondary B6D2F1 recipients together with TCD-BM from naïve B6 mice. The secondary recipients were left untreated after transfer. (A) A schematic overview of the experiments. (B) Change in body weight after the transfer (n = 5 per group). (C) H&E staining of the skin (original magnification ×200; scale bar, 100 μm), and MT staining of the salivary glands (priginal magnification ×200; scale bar, 100 μm) and liver (original magnification ×40; scale bar, 500 μm) on day 50 after transfer. Proportions of fibrotic area to the whole section area in the salivary glands (D) and liver (E). (F) The volume of tear secretion was measured on day 50 after the transfer. (G-N) The first allo-HCT was performed as in Figure 1A and mice were administered with CSP from days 0 to 14 after allo-HCT. A total of 2 × 106 Ly6C+ (transitory-Tex-CNI) or Ly6C– (terminal Tex) donor T cells were sorted from CSP-treated recipients on day 14 and adoptively transferred into irradiated secondary recipients together with TCD-BM from naïve B6 mice. (G) A schematic overview of the experiments. Representative contour plots (H) and proportions (I) of Ly6C expression on donor T cells before or 50 days after the transfer of terminal Tex and transitory-Tex-CNI (n = 5 per group). (J) Expression of indicated molecules in Ly6C+ and Ly6C– CD4+ (top) and CD8+ (bottom) T cells in the spleen on day 50 after the adoptive transfer of transitory-Tex-CNI. (K) Change in body weight after adoptive transfer (n = 5 per group). Fibrotic area in the salivary glands (L) and liver (M), and volume of tear secretion (N) were evaluated 50 days after the adoptive transfer. (B,I-K) Data from one of 2 similar experiments were shown as means ± SEM. (D-F, L-N) Data from 2 independent experiments were combined and shown as means ± SEM. ∗P < .05; ∗∗P < .01; ∗∗∗P < .005. ns, not significant.

Transitory-Tex-CNI contributed to the development of cGVHD. (A-F) The first allo-HCT was performed as in Figure 1A, and recipient mice were orally administered with CSP or vehicle from days 0 to 14 after allo-HCT. A total of 1 × 106 T cells purified from vehicle- or CSP-treated recipients 14 days were adoptively transferred into lethally irradiated secondary B6D2F1 recipients together with TCD-BM from naïve B6 mice. The secondary recipients were left untreated after transfer. (A) A schematic overview of the experiments. (B) Change in body weight after the transfer (n = 5 per group). (C) H&E staining of the skin (original magnification ×200; scale bar, 100 μm), and MT staining of the salivary glands (priginal magnification ×200; scale bar, 100 μm) and liver (original magnification ×40; scale bar, 500 μm) on day 50 after transfer. Proportions of fibrotic area to the whole section area in the salivary glands (D) and liver (E). (F) The volume of tear secretion was measured on day 50 after the transfer. (G-N) The first allo-HCT was performed as in Figure 1A and mice were administered with CSP from days 0 to 14 after allo-HCT. A total of 2 × 106 Ly6C+ (transitory-Tex-CNI) or Ly6C– (terminal Tex) donor T cells were sorted from CSP-treated recipients on day 14 and adoptively transferred into irradiated secondary recipients together with TCD-BM from naïve B6 mice. (G) A schematic overview of the experiments. Representative contour plots (H) and proportions (I) of Ly6C expression on donor T cells before or 50 days after the transfer of terminal Tex and transitory-Tex-CNI (n = 5 per group). (J) Expression of indicated molecules in Ly6C+ and Ly6C– CD4+ (top) and CD8+ (bottom) T cells in the spleen on day 50 after the adoptive transfer of transitory-Tex-CNI. (K) Change in body weight after adoptive transfer (n = 5 per group). Fibrotic area in the salivary glands (L) and liver (M), and volume of tear secretion (N) were evaluated 50 days after the adoptive transfer. (B,I-K) Data from one of 2 similar experiments were shown as means ± SEM. (D-F, L-N) Data from 2 independent experiments were combined and shown as means ± SEM. ∗P < .05; ∗∗P < .01; ∗∗∗P < .005. ns, not significant.

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