CSP induced donor transitory-Tex-CNI after allo-HCT. (A-L) Allo-HCT was performed as in Figure 1A. Mice were treated with vehicle or CSP daily from days 0 to 28 after allo-HCT. FCM of donor T cells in the spleen were performed. Contour plots (A) and proportions (B) of PD-1 and TIGIT expression, and absolute numbers of donor PD-1⁺TIGIT⁺ (C) and PD-1⁺TIGIT^– (D) cells in 2C-Tg donor T cells (n = 6 per group). (E) MFI of GZMB in 2C-Tg PD-1⁺TIGIT⁺ or PD-1⁺TIGIT^– cells on days 14 and 28 after allo-HCT (n = 6 per group). (F) Contour plots of TOX and TCF-1 expression in 2C-Tg donor T cells on day 14 after allo-HCT. MFI of TOX (G) and absolute numbers of TOX^low cells (H) (n = 6 per group). (I) Contour plots of TOX and Ly6C expression in 2C-Tg donor T cells. Proportions (J) and absolute numbers (K) of Ly6C⁺ cells in 2C-Tg donor T cells (n = 5 per group). (L) MFIs of PD-1, TIGIT, TOX, TCF1, Ki-67, and GZMB in Ly6C⁺ or Ly6C^– donor 2C-Tg T cells in CSP-treated recipients (n = 5 per group). (B-E, G-H) Data from 2 experiments were combined and shown as means ± SEM. (J-L) Data from one of 2 similar experiments were shown as means ± SEM. ∗P < .05; ∗∗P < .01; ∗∗∗P < .005. MFI, mean fluorescence intensity; ns, not significant.