American Society of Hematology

Figure 3.

$The disorders of the BM environment in 9p21s+/− MDS/MPN–like mice. (A) Hematoxylin and eosin and reticulin staining of serial femur bone sections from a representative 9p21+/− MDS/MPN mouse. (B) Micro-CT images of the femurs in 9p21+/− tumor and WT mice. Red arrows show the abnormal cortical bone in 9p21+/− tumor mice. (C) Quantification of trabecular bones and cortical bones volume in femur and tibia total bone volume. Symbols represent individual mice (WT mice, n = 5; 9p21+/− tumor mice, n = 6). Data represent the mean ± SD. Statistical significance was defined using Mann-Whitney test and is shown as follows: ∗∗P < .01. (D) Hematoxylin and eosin staining of knee joints from 9p21+/− tumor and WT mice. Green dotted lines indicate increased fibrous regions in meniscus; gold arrows indicate bone formation in meniscus; a blue dotted line indicates thicker synovium; red arrows indicate osteophyte in cartilage. Ct. BV/TV, cortical bone volume fraction; Ct.Th, cortical bone thickness; F, fibrous region; Fem, femur; M, meniscus; S, synovium; Tb. BV/TV, trabecular bone volume fraction; Tib, tibia.$

The disorders of the BM environment in 9p21s^+/− MDS/MPN–like mice. (A) Hematoxylin and eosin and reticulin staining of serial femur bone sections from a representative 9p21^+/− MDS/MPN mouse. (B) Micro-CT images of the femurs in 9p21^+/− tumor and WT mice. Red arrows show the abnormal cortical bone in 9p21^+/− tumor mice. (C) Quantification of trabecular bones and cortical bones volume in femur and tibia total bone volume. Symbols represent individual mice (WT mice, n = 5; 9p21^+/− tumor mice, n = 6). Data represent the mean ± SD. Statistical significance was defined using Mann-Whitney test and is shown as follows: ∗∗P < .01. (D) Hematoxylin and eosin staining of knee joints from 9p21^+/− tumor and WT mice. Green dotted lines indicate increased fibrous regions in meniscus; gold arrows indicate bone formation in meniscus; a blue dotted line indicates thicker synovium; red arrows indicate osteophyte in cartilage. Ct. BV/TV, cortical bone volume fraction; Ct.Th, cortical bone thickness; F, fibrous region; Fem, femur; M, meniscus; S, synovium; Tb. BV/TV, trabecular bone volume fraction; Tib, tibia.

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