Figure 5.
Engineered t(5;14) is not sufficient for TLX3 activation. (A) Schematic overview of the experimental strategy for CRISPR-mediated genome editing to generate t(5;14) isogenic Jurkat cells using UT242-associated chromosomal break points. JurkatWT indicates parental cell line, whereas JurkatSV indicates t(5;14)-edited clonal cell line. (B) Detection of t(5;14) in DND-41 and Jurkat cells by DNA FISH on metaphase chromosomes. Dotted boxes and arrows mark t(5;14) alleles. (C) Expression of BCL11B and TLX3 mRNA in parental and edited Jurkat cells normalized to GAPDH. Results are mean ± SD (N = 6 biological replicates) and analyzed by a Student t test. ∗∗∗P < .001, ∗∗∗∗P < .0001, n.s. not significant. (D) Genome browser tracks for chr5 and chr14 showing ATAC-seq and 4C-seq in parental and edited Jurkat cells. Coordinates correspond to hg19 genome assembly. The purple arrowhead indicates the 4C-seq viewpoint.

Engineered t(5;14) is not sufficient for TLX3 activation. (A) Schematic overview of the experimental strategy for CRISPR-mediated genome editing to generate t(5;14) isogenic Jurkat cells using UT242-associated chromosomal break points. JurkatWT indicates parental cell line, whereas JurkatSV indicates t(5;14)-edited clonal cell line. (B) Detection of t(5;14) in DND-41 and Jurkat cells by DNA FISH on metaphase chromosomes. Dotted boxes and arrows mark t(5;14) alleles. (C) Expression of BCL11B and TLX3 mRNA in parental and edited Jurkat cells normalized to GAPDH. Results are mean ± SD (N = 6 biological replicates) and analyzed by a Student t test. ∗∗∗P < .001, ∗∗∗∗P < .0001, n.s. not significant. (D) Genome browser tracks for chr5 and chr14 showing ATAC-seq and 4C-seq in parental and edited Jurkat cells. Coordinates correspond to hg19 genome assembly. The purple arrowhead indicates the 4C-seq viewpoint.

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