ERFE overexpression does not alter blood erythrocyte parameters in adult thalassemic mice. Mice generated from Th3 × E(M) breedings were analyzed at 16 weeks. Measurements in males are depicted in closed circles and females in open circles. (A-C) Erythroferrone levels were assessed by (A) BM Erfe mRNA, (B) spleen Erfe mRNA levels, (C) serum ERFE. (D) Serum EPO. (E-J) Erythrocyte parameters: (E) Hb levels, (F) RBC count, and (G) hematocrit. (H-J) Body and organ weights: (H) total body weight, (I,J) liver and spleen weights as a percentage of total body weight. P values were assessed by two-tailed unpaired t test (∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001) between the Th3 and T-E(M) groups only and (A-C) also between E(M) and T-E(M).