CD45RA-depleted DLI induce hyperexpansion of selected T-cell clones. TCR-seq was performed on samples of patients who received DLI collected at baseline and T7 (n = 10 patients), the DLI (n = 8), and samples of control patients (n = 10 patients) collected around day +50 and day +150. (A) Left graph shows the Chao1 diversity index and right graph shows the Gini diversity index. Medians are indicated. Statistical significance was determined by Wilcoxon test. (B) Clonotypes were categorized into small, medium, large, and hyperexpanded clones which constituted <0.01%, 0.01%–0.1%, 0.1%–1%, and >1% of the repertoire, respectively. Medians are indicated. Statistical significance was determined by Wilcoxon test.