T- and NK-cell dynamic changes after haplo-HSCT with CD45RA-depleted DLI. (A-C) Total CD8⁺ T cells (A), CD4⁺ T cells (B) and NK cells (C) were clustered by PhenoGraph. Cluster identities are revealed by balloon plots, whereas the heatmaps show median cluster dynamics in patients who received DLI (n = 19) at different time points, their donors (n = 18), and unrelated CMV⁺ healthy controls (n = 12). Samples containing <50 total CD8⁺ T cells, CD4⁺ T cells, or NK cells were excluded from temporal analysis. Act, activated; HC, healthy control; T_CM, central memory T cell; T_EF, effector T cell; T_EM, effector memory T cell; T_EMRA, effector memory T cell re-expressing CD45RA; T_M, memory T cell; T_N, naïve T cell; T_REG, regulatory T cell; T_TE, terminal effector T cell; T_TM, transitional memory T cell.