SECTM1 CAR T cells eradicated CD7⁺ primary T-ALL cells. (A) CD7 expression level of primary leukemic blasts from 4 patients with T-ALL, the percentage of CD7⁺ cells were showed compared with ISO. (B) The remaining live tumor cells were calculated to detect the cytotoxicity of SECTM1 CAR T cells against T-ALL primary leukemic blasts. (C) IFNγ and TNFα released by the CAR T cells in the experiment described in panel B was detected by ELISA. Left, the IFNγ level; right, the TNFα level. Data in panels B and C are presented as the mean ± SD of triplicates.