Lysis of CD7⁺ ALL and AML cells by SECTM1 CAR T cells. (A) CD7 expression in ALL cell lines (CCRF-CEM and MOLT4), and AML cell lines (KG-1a and Kasumi-3) was analyzed by flow cytometry. (B) Tumor cells were cocultured with CAR T cells at various E:T ratios, and cytotoxicity was measured at 20 hours by flow cytometry. (C) Cytokines released into the coculture supernatant described in panel B of an E:T ratio of 2:1 was detected by ELISA. Left, the IFNγ level; right, the TNFα level. Data in panels B and C are presented as the mean ± SD of triplicates.