A-485 drives gene expression changes through remodeling enhancer landscape in LSC. (A) Heatmap of expression changes of the genes obtained from LSC treated with A-485 (500 nM). Selected LSC enhancer–associated genes are labeled. (B) Gene ontology analysis of gene expression changes in LSC cells treated with A-485. (C) Volcano plots of differential H3K27ac peaks in LSC treated with DMSO and A-485, revealing 3010 differential peaks for H3K27ac in A-485-treated vs DMSO-treated LSCs. (D) Top enriched GO terms for significantly downregulated H3K27ac peaks in LSC treated with A-485 determined by querying the Molecular Signatures Database (MSigDB) Perturbation genesets. (E) Motif analysis of downregulated H3K27ac ChIP-seq peaks that overlap with downregulated genes. (F,G) Aggregation plots showing average ChIP-seq signals for H3K27ac and EP300 marks in LSC treated with A-485 for various times at enhancer regions. Plots are peak-centered and scaled at ±3 kb for each locus.DMSO, dimethyl sulfoxide.