FOXP1 regulates AML cell resistance to chemotherapy. AML cells were transduced or not (−), with control (Ctl) shRNA- or shFOXP1-expressing vector (#b). Daunorubicin (DNR) was added 2 days later for 24 hours; (A) alive cells were identified by flow cytometry forward and side scatter fluorescence and expressed as percentage of total cell events; difference between control shRNA- and shFOXP1#b-expressing cells were analyzed for significance using a Mann-Whitney test (n = 4). (B) Cells were lysed and protein expression was assessed using western blot, with GAPDH as a loading control. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.