FOXP1 limits oxidative stress of AML cells. (A) Kaplan-Meier survival curves of patients with CN-AML (GSE12417; n = 163) based on FOXP1 gene expression levels, using median expression value cutoff (left) or first and third quartile statistical analysis (right). Log-rank (Mantel-Cox) test was used for statistical significance between groups. (B-C) MOLM-14 or (D) primary human FLT3-ITD AML cells (supplemental Table 3) were transduced with the indicated GFP- and shRNA-encoding lentiviral vector. After 3 days, oxidative stress was assessed in viable GFP⁺ cells using a DHE probe and flow cytometry (B,D) (n = 4). In parallel, indicated protein (B) (bottom) and RNA transcript (C) (n = 5 or 6) expression levels were analyzed. (BD) Data are expressed as MFI arbitrary unit (a.u); (C) normalized RNA transcript levels were expressed relative to mean level of control shRNA-expressing cells (n = 9); 1 spot represents 1 independent sample from independent experiments. − indicates no vector. Ctl, control shRNA directed against luciferase; MFI, mean fluorescence intensity.