Placental and fetal iron homeostasis is dysregulated in dams carrying Th3/+ fetuses. (A) Placental weight was increased in Th3/+ dams. (B) Placental NHI. (C) Fetuses from thalassemic dams weighed less than those from WT dams, and (D) placental weight normalized by fetal weight was increased in Th3/+ dams. (E) Fetal liver iron and (F) liver hepcidin mRNA expression were elevated in fetuses from WT2 and Th3/+ group dams. Moreover, FPN1 expression was quantified in the placentas. (G) FPN1 expression was normalized to expression of β-actin, which did not vary significantly among experimental groups. Results represent mean ± SD for n = 5 mice per group and were analyzed by one-way ANOVA followed by Tukey multiple comparisons test. Groups labeled with different letters are significantly different from one another (P < .05). Significant main effects: panels A-E,G, P < .001; panel F, P < .0001.