Expression of iron transport- and storage-related proteins in Th3/+ vs WT dams. Expression of DMT1, FPN1, and FTH in the intestine (A-D), the liver (E-I), and the spleen (J-M) was quantified by western blotting. Furthermore, because the liver acquires iron from circulating transferrin, hepatic TFR1 expression was also quantified (E,I). Expression of each protein was normalized to the expression of β-actin. Results represent mean ± SD for n = 4 mice (WT2) or n = 5 mice per group (WT1 and Th3/+) and were analyzed by one-way ANOVA followed by Tukey multiple comparisons test. Groups labeled with different letters are significantly different from one another (P < .05). Significant main effects (from P < .05-P < .0001) noted for all quantification data for panels B-M, except for intestinal FTH (D).