Selected iron (⁵⁹Fe) absorption into organs of pregnant WT and Th3/+ mice. (A) Intestinal iron absorption was quantified after oral, intragastric gavage of an ⁵⁹Fe test dose, and presented as the percentage of radioactivity present in the animals (minus ⁵⁹Fe trapped in the gut) 24 hours after dosing. Also shown is the distribution of ⁵⁹Fe in the liver (B) and the spleen (C), which is presented as the percentage of absorbed ⁵⁹Fe in each tissue. Results represent mean ± SD for n = 5 animals per group and were analyzed by one-way ANOVA followed by Tukey multiple comparisons test. Groups labeled with different letters are significantly different from one another (P < .05). Main effects: panels A-C, P < .0001. ⁵⁹Fe accumulation in other tissues and that remaining in the carcasses after dissection of all other tissues are presented in Table 4.