Effects of pregnancy on selected iron parameters in WT and Th3/+ mice. NHI levels in the liver (A), spleen (B), and kidney (C), and serum ferritin concentrations (D). Data represent mean ± SD for n = 6 mice per group (WT1 and Th3/+) or n = 5 mice per group (WT2, NP-WT, and NP-Th3/+). Data were analyzed by one-way ANOVA followed by Tukey multiple comparisons test. Groups labeled with different letters are significantly different from one another (P < .05). Significant main effects: panels A-D, P < .0001.