EPO-ERFE-Hamp signaling axis in dams of both genotypes. (A) Serum EPO levels, (B) relative bone marrow ERFE mRNA expression levels, and (C) serum hepcidin concentrations. (D) Serum hepcidin is low relative to iron load (Figure 3A) in Th3/+ dams and in WT dams carrying Th3/+ fetuses (WT2 group). Data represent mean ± SD for n = 5 (WT2, NP-WT, and NP-Th3/+) or 6 (WT1 and Th3/+) mice per group and were analyzed by one-way ANOVA followed by Tukey multiple comparisons test. Groups labeled with different letters are significantly different from one another (P < .05). Main effects: panels B-D, P < .01; panel A, P < .001.