Effect of pregnancy on hematological and iron-related blood parameters in dams of both genotypes. (A) Hb levels; (B) RBC count; (C) reticulocyte count; (D) serum NHI; (E) TSAT; (F) TIBC. Data represent mean ± standard deviation (SD) for n = 6 mice per group (WT1 and Th3/+) or n = 5 mice per group (WT2, NP-WT, and NP-Th3/+). Data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey multiple comparisons test. Groups labeled with different letters are significantly different from one another (P < .05). Significant main effects: panels A-F, P < .0001.