Mathematical model of clonal loss and stochastic HSC engraftment. (A) Overlay of observed clonal loss in Z13264 (red line) and Z14004 (green line) with the results of our mathematical model of loss of unique clones (black lines, Nt for t = 1 ,….., T = 1000 days). Different starting cell numbers of engrafted HSCs were explored in the simulations as indicated. (B) CITE-seq of a representative human steady-state BM CD34+ population. (Left) Transcriptionally distinct progenitor subsets were color coded as indicated in the color key. (Right) Cell-cycle status of cells. (C) Longitudinal change of clonal loss and HSC clone size. Simulations were performed with 120 000 HSCs at the time of transplant, an initial cell-cycle rate of 100% per day for 20 days and 10% cycle rate per day thereafter. The number of unique clones for each representative time point is shown (bottom right). Dot sizes illustrate the clone sizes.
