Contribution and persistence of multipotent 5-lineage clones. (A) Summary of retrospectively assigned lineage contribution for unique clones. (B) Longitudinal detection of multipotent 5-lineages clones. Blue and gray indicate the presence and absence of clones, respectively. Graph below heatmap: frequency of 5-lineage clones detected (blue, left y-axis) and number of reads (green, right y-axis) for each time point. BM time points are outlined in red. Time points with multiple FACS-purified lineages are highlighted in yellow. (C) Longitudinal contribution of 5-, 4-, 3-, 2-, and 1-lineage clones to the PB. Color code as defined in panel A. (D) Impact of sampling on the reliability of IS detection. Correlation of the cumulative read count from a single PB (black symbol) or BM (red symbol) time point, with the frequency of multipotent 5-lineage clones detected at the same time point. (E) Impact of clonal abundance on the reliability of IS detection. Correlation of the mean normalized abundance of 5-lineage clones (black dots), with the number of times the clone was detected across all analyzed samples. Gray background indicates clones associated with other groups. The mean normalized abundance and average number of times detected across all clones are indicated with the green horizontal line and the blue vertical line, respectively.