Increased levels of RBC-derived EVs in patients with PIEZO1 GoF mutations. (A) Flow cytometry analysis for RBC-derived EVs. Glycophorin A or CD235a, y-axis, and CD47 (x-axis) (axis values in log scale). Window P2 has CD235a⁺/Cd47⁺ cell populations that represent RBC-derived EVs. The analysis was performed in blood samples from HCs and 2 patients with DHS with PIEZO1 mutations (DHS17 and DHS18), not treated or treated with Yoda1 (PIEZO1 activator) and CaCl₂. (B) RBC-derived EVs numbers in samples from flow cytometry analysis shown in panel A.