Validation analysis of the transport, vesicle, and endocytosis pathways by multiple-reaction monitoring analysis. (A) Graphic representation obtained by the GraphPad software of the log2 normalized mean areas for all the proteins validated in patients with DHS (red boxes) compared with HCs (blue boxes). (B) Left: expression levels of OSR1 protein in patients with DHS with mutations in the mechanosensing module (green boxes) compared with patients with transduction module/ion-conducting pore mutations (purple boxes). Right: phosphorylation state analysis of OSR1 peptide (from amino acid 185-202) phosphorylated and nonphosphorylated in patients with DHS with mutations in the mechanosensing module (green boxes) compared with patients with transduction module/ion-conducting pore mutations (purple boxes). The representations were obtained by GraphPad software. A 2-way ANOVA test in a multiple comparison way was applied; ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; and ∗∗∗∗P ≤ .0001.