The effects of selinexor on the nuclear export of critical proteins. EV- or eIF4E-overexpressing H929 cells were treated without or with selinexor. Cytoplasmic and nuclear fractions were extracted followed by WB using antibodies against XPO1, eIF4E, IKZF1, and c-MYC. LaminB1 and β-actin were blotted as the markers for nuclear and cytoplasmic fractions, respectively. (Right) Band density analysis of eIF4E in cytoplasmic fractions. eIF4E protein levels were quantified using NIH ImageJ. Values represent the mean ± SEM for 2 independent experiments.