Overexpression of eIF4E induces resistance to selinexor. (A) eIF4E was overexpressed in H929 MM cells and confirmed using WB. The densities of the bands were measured using NIH ImageJ and normalized for β-actin. The immunoblot is representative of 4 independent experiments. (B) EV- or eIF4E-overexpressing (eIF4E) H929 cells were treated with different doses of selinexor, and cell proliferation was analyzed. ∗P < .05. Quantification of the maximal inhibitory effect of selinexor on H929 proliferation is shown. (C) EV- or eIF4E-transduced H929 cells were treated with selinexor and evaluated via cell apoptosis assay. (D) EV- or eIF4E-transduced H929 cells were treated with selinexor followed by assessment with a cell cycle assay. Right panel values represent the mean ± SEM for 2 independent experiments. SEM, standard error of the mean.