Inhibitory effects of the addition of FVa mutants to anticoagulant-deficient plasmas, determined with the diluted-PT reagent-based assay. Diluted PT reagent was added to (A) pooled normal, (B) AT-deficient, (C) PC-deficient, and (D) PS-deficient plasma, and mixed with generated FVa mutant samples (f.c. 4.5 nM), followed by the addition of CaCl₂. Clotting times were measured in seconds as described in “Methods.” Experiments were performed at least 3 times, and the average values ± standard deviations are shown. One-way analysis of variance was performed on experimental data.