Extensive dysregulation of proteins required for cell cycle and associated processes, DNA repair, and membrane trafficking in KLF1^+/E325K cells. (A) Z-scored scaled abundance values for proteins associated with cell cycle (Gene Ontology database GO:0007049) and (B) chromosome segregation (GO:0098813) in WT and KLF1^+/E325K cells on day 2 of differentiation. (C) Quantification of western blots for WT, KLF1^+/E325K, and KLF1^+/− cells during expansion (day 0) and on days 2, 4, and 6 of differentiation probed with antibody to γH2AX (mean ± SD; n = 3; ∗∗∗P < .001 compared with that for WT), and (D) representative example of γH2AX western blot, with β-actin as loading control. (E) Western blots of WT, KLF1^+/E325K, and KLF1^+/− cells on day 2 of differentiation probed with antibodies to RAD51, SIRT6, DROSHA, and to β-actin as a loading control. Each blot is representative of 3 biological repeats. (F) Z-scored scaled abundance values for proteins associated with DNA repair (GO:0006281) in WT and KLF1^+/E325K cells on day 2 of differentiation. (G) Heat maps of z-scored abundance values for exocyst complex and (H) kinesin superfamily (KIF) proteins in WT and KLF1^+/E325K cells on day 2 of differentiation. (I) Z-scored scaled abundance values for proteins associated with vesicle tethering complex (GO:0099023) in WT and KLF1^+/E325K cells on day 2 of differentiation. (J) (i) Heat maps of z-scored abundance values for TMED proteins in WT and KLF1^+/E325K cells on day 2 of differentiation (n = 3) and (ii) western blot for the selected TMED, TMED10. For heat maps, each box represents 1 biological repeat (total n = 3).