Overexpanded myeloid-biased Tet2 KO clones drive MPP3 and granulocyte expansion. (A) Hierarchical clustering of all clones from control and Tet2 KO mice identifies 3 major clusters. Clones in each cluster are grouped by Tet2 genotype and expansion profiles. Overexpanded clones were determined as shown in Figure 2A. (B) The average clonal abundance of each cell type in the 3 major clusters from (A). Error bars represent standard deviation. (C) Expected and observed number of Tet2 KO overexpanded clones in each cluster. The expected clone numbers are calculated based on the frequency in each cluster of all detected clones from the control and KO groups. χ2 = 20.5; P < .0001. (D) Number of unique clones across the 3 clusters. (E) HSC and MPP3 clonal abundance in the BM and granulocyte clonal abundance in the peripheral blood of control and Tet2 KO normally expanded and Tet2 KO overexpanded clones across the 3 clusters. Bars represent the median. Two-tailed Mann-Whitney U test. ns: not significant. ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.
