Preleukemic clonal expansion in Tet2 KO mice is driven by rare, overexpanded clones. (A) Tet2 KO overexpanded clones are defined as clones whose abundances are greater than the most abundant clone in control mice. The blue bar shows the average abundance of the most abundant clone from each of the 4 control mice, which was used as the threshold to identify overexpanded clones. Each dot represents 1 overexpanded clone. (B) The number of Tet2 KO overexpanded clones in each cell type. (C) Tet2 KO overexpanded clones across cell types. Each column represents 1 unique clone. Some clones are identified as overexpanded in multiple cell types. (D) Hierarchical clustering of Tet2 KO overexpanded clones based on their abundance in different cell types. (C-D) Clones identified from the same mouse are indicated as a unique color (top). (E) Numbers and abundances of Tet2 KO overexpanded clones among all Tet2 KO clones. (F) Comparing HSC and MPP3 clonal abundance in the BM and granulocyte clonal abundance in the peripheral blood of control, Tet2 KO normally expanded and overly expanded clones. White dots represent the median. Two-tailed Mann-Whitney U test. ∗∗∗∗P < .0001. (G) The clonal abundance of granulocytes in the peripheral blood before population-level expansion is detected. Shaded regions represent SEM.