Characterization of the chromatin and transcriptional features of regions de novo acetylated in subset 8 CLLs. (A) Heatmap of the acetylation levels of the 209 de novo active regions including 4 U-CLL cases who experienced Richter transformation with multiple time points (diagnosis, progression, relapse, and Richter transformation). (B) A total of 9 TF binding sites (TFBSs) show statistically significant enrichment (y-axis) in cluster 1 regions and significant correlation with gene expression. The color scale represents the significance of the differences in the expression levels between subset 8 and U-CLL. The left panel shows the binding motifs for each TF. The red lines highlight the cut-off for the statistically significant results in each analysis. (C) Hierarchical clustering is based on 209 regions and associated genes. The heatmap on the left contains the H3K27ac values, whereas that on the right shows the expression values of the associated gene. As some peaks are associated with more than 1 gene, rows of the left heatmap can be duplicated. (D) Barplots showing the H3K27ac peaks that were correlated with the expression levels of more than 2 genes. (E) Hi-C map for 1 representative nonstereotyped U-CLL case and genome browser display of TAD1791 containing peak-61315 (including also the flanking TADs 1790 and TAD1792 as controls). We show the H3K27ac coverage plot of a representative subset 8 and nonstereotyped U-CLL. On the left, a zoom-in panel of the significant peak-61315 within TAD1791 including a boxplot of the H3K27ac levels and a heatmap with the gene expression levels of the 7 associated genes.