Platelet-specific PDK2/4 contributes to arterial thrombosis. (A) The left panel demonstrates the schematic depicting the technique for generating mice with platelet-specific PDK2 and PDK4 deficiency. Thrombocytopenia was induced in hIL-4Rα/GPIbα-Tg mice by injecting antibodies against human IL-4R and platelet count was measured after 2 hours. N = 5 mice per group. Statistical analysis: Mann-Whitney U test; ∗∗P < .01. This was followed by the adoptive transfer of WT or PDK2/4^−/− platelets into hIL-4Rα/GPIbα-Tg mice. (B) The left panel shows a representative image of carotid artery thrombus (5% FeCl₃ injury for 3 minutes) as visualized by intravital microscopy in hIL-4Rα/GPIbα-Tg male mice transfused with WT or PDK2/4^−/− platelets. The right panel shows the time to occlusion. The time to occlusion was measured until 40 minutes, the cutoff point at which the experiment was terminated. Values are mean ± SEM, n = 8 vessels from 4 mice per group. Data represents left and right carotid artery injury from each mouse. Statistical analysis: Mann-Whitney U test; ∗P < .05.