Clinical outcomes of patients receiving cryopreserved versus fresh PBSCs. PFS (A), NRM (B), and relapse (B) were similar between patients receiving fresh (blue or green) versus cryopreserved (red or orange) PBSCs. Incidence of all grade cGVHD and moderate/severe cGVHD was lower in patients receiving cryopreserved PBSCs (red or orange) compared with those receiving fresh PBSCs (blue or green) (C). This difference was only observed in patients treated with tacrolimus+methotrexate +/− sirolimus for GVHD prophylaxis (D) but not in patients given PTCY for GVHD prophylaxis (E).