Coexpression of TIM-3 and PD-1 on CD4⁺T-cells in patients with CML and in HD. (A) PD-1⁻TIM-3⁺CD4⁺ T cells as a percentage of CD4⁺ T cells were significantly higher in both the patients in MolR group and those in TFR group than in healthy donors (HD). Furthermore, the HD had lower expression of TIM-3 than patients in the TFR group. (B) The absolute counts of PD-1⁻TIM-3⁺CD4⁺ T cells was higher in patients in the MolR group than those in the TFR group. (C) PD-1⁺TIM-3⁻CD4⁺ T cells as percentage of CD4⁺ T cells or (D) absolute counts of PD-1⁺TIM-3⁻CD4⁺ T cells were not different between the groups. (E) PD-1⁺TIM-3⁺CD4⁺ T cells as a percentage of CD4⁺ T cells was higher in the patients who experienced MolR than in those with sustained TFR and in HD. (F) Absolute counts of PD-1⁺TIM-3⁺CD4⁺ T cells were significantly higher in the MolR group compared with the TFR group. (G) Conversely, PD-1⁻TIM-3⁻CD4⁺ T cells were significantly lower in the MolR group compared with both the TFR and HD group. (H) There was no difference in the absolute counts of PD-1⁻TIM-3⁻CD4⁺ T cells. The Mann-Whitney test was used to compare 2 groups, whereas multiple comparisons were made using the Kruskal-Wallis test; alpha was set at 0.05 (adjusted for multiple comparisons).