Mnk1 deficiency reduces platelet activation. (A-B) Washed platelets from WT and Mnk1 KO mice were left unstimulated (US) or were stimulated with thrombin (0.05 and 0.5 U), collagen (2 and 10 μg/mL), and 2MeSADP (2 and 25 nM). The curves are representative of n = 3 independent experiments. The bar graphs on the right show quantitation of platelet aggregation (n = 3 mice per group; ∗P < .05). (C-D) Flow cytometric analyses of JON/A binding (for activated integrin αIIbβ3) or P-selectin surface expression on washed platelets from WT and Mnk1 KO mice following stimulation with thrombin (0.05 and 0.5 U), collagen (2 and 10 μg/mL), and 2MeSADP (2 and 25 nM). Graphs show data from n = 3 mice per group (∗P < .05). NS, not significant.