ΔdblGATA mice are resistant to EAE. (A) WT and ΔdblGATA mice were immunized for EAE induction. Representative data showing the clinical course from 1 of >5 experiments. Significance was calculated using 2-way analysis of variance (ANOVA). (B) Change in body weight over time. Significance was calculated by comparing the means from each day. (C) Quantification of the maximum clinical score. (D) Weight loss relative to the starting weight on the day of immunization. Significance for maximum clinical score and weight loss were calculated using Student t test. (E) Disease incidence. Significance was calculated using χ² test. N = 24 to 25 per group in panels C-E. (F) Luxol fast blue (LFB) and hematoxylin and eosin (H&E) staining for myelin in the spinal cord sections. (G) Quantification of inflammation, calculated as percent area of white matter occupied by cell infiltration. N = 2 to 3 per group. (H-I) Adoptive EAE in WT and ΔdblGATA mice. WT and ΔdblGATA mice were immunized with the PLP_180-199 peptide, euthanized on day 8 after immunization, and splenic cells were stimulated with the peptide for 3 days in vitro. Then, 10⁷ of purified CD4⁺ T cells from WT and ΔdblGATA mice were transferred to naïve WT and ΔdblGATA recipients. One of 2 independent repeats is shown. (H) Clinical score. (I) Change in body weight for panel H is shown. Statistical significance for panels H and I was calculated by 2-way ANOVA. Max., maximum.