Increased vesiculation of MC uRBCs and condition medium–exposed RBCs is associated with laminin-α5 interaction. (A) Microvesicles were characterized as Hoechst-negative cells corresponding to a low side scatter (SSC) and were quantified in control, malaria culture (MC), and conditioned-medium (CM) samples. Selected fluorescence-activated cell sorting (FACS) plots are representative of 8 to 10 biological replicates. (B) Flow cytometric quantification of microvesicles in control RBCs (control) and in MC RBCs. n = 8. (C) Percentage of microvesicles in control RBCs (control) and in CM-exposed RBCs. n = 8. Wilcoxon matched-pairs signed rank test. (D) Percentage of microvesicles in samples from patients with malaria and age-matched controls. Mean ± SD; n = 5-6; Mann-Whitney test. (E) Mean florescence intensities (MFI) of Fluo-4–stained uRBCs from CM-incubated RBCs (y-axis) were plotted against the percentage of microvesicles (x-axis; n = 18); 95% confidence interval, 0.3841-0.8890.