TCR repertoire diversities of naive CD8⁺and CD4⁺T cells are increased after the T-cells expansion induced by hIL-7-hyFc. (A) Gating strategy for isolating the naive and memory subsets of CD8⁺ and CD4⁺ T cells by flow cytometry. Each subset was isolated on days 0 and 56 from 4 individuals in cohort 3 (n = 4) or was isolated on day 0 and post (day 21-56) from 4 individuals in cohort 1 and 2 (n = 2 from each cohort, respectively). The TCR repertoire diversity of each isolated subset was analyzed by sequencing the TCR_β CDR3 regions. (B) Inverse Simpson index, (C) normalized Shannon index, and (D) U/T index are shown. (E) V(D)J gene usage of CDR3 regions of naive CD8⁺ T cells on day 0 (top) and day 56 (bottom), shown as VJ gene combinations. Yellow boxes with “X” indicate VJ gene combinations with undetected use. Bright red boxes indicate VJ gene combinations with >1% usage of total combinations. V or J genes not shown on the horizontal or vertical axis were excluded because they were not detected in CDR3 sequencing. P values were calculated with a Wilcoxon matched-pairs signed-rank test. ∗P < .05.