hIL-7-hyFc increases the absolute numbers of CD8⁺and CD4⁺T-cell subsets. (A) Gating strategy for obtaining flow cytometry-based frequencies of the T_N, T_EM, T_CM, T_EMRA, T_SCM subsets, and CD4⁺ RTEs. (B-C) FC over baseline for the absolute counts of the T_N, T_EM, T_CM, and T_EMRA subsets among CD8⁺ T cells (B) and CD4⁺ T cells (C). T_N, T_EM, T_CM, and T_EMRA subsets are shown in order from top to bottom. Black asterisks above the graphs indicate P values comparing the value of each time point with that of baseline (day 0) by a Wilcoxon matched-pairs signed-rank test. (D) Absolute numbers of the T_SCM subsets among CD8⁺ T cells (top) and CD4⁺ T cells (bottom) on days 0, 21, and 56. Values shown are the mean (± standard error of the mean) for cohorts and placebo at the specified times. P values between the value of each time point in each cohort were calculated with a Wilcoxon matched-pairs signed-rank test. The statistical significance between the cohorts were calculated with a two-way RM ANOVA with Geisser-Greenhouse correction. ∗∗P < .01, ∗P < .05.