Figure 4.
IGF2BP1 and IGF2BP3 mediate the HbF silencing activity of BMI1 in primary adult erythroblasts. (A) Schematic of BMI1 depletion experiments in primary adult erythroid cells. (B-C) Volcano plots of DEGs identified from RNA-seq in BMI1-depleted primary adult erythroblasts (sgBMI1#1 or sgBMI1#3) by DESeq2. Cutoff threshold: fold change > 2; FDR < 0.1; n = 2. (D) Venn diagram of overlapped DEGs identified from RNA-seq in BMI1-depleted primary adult erythroblasts by comparing control sample with sgBMI1#1 (B) and sgBMI1#3 (C) sample, respectively. (E) Representative immunoblots of BCL11A, IGF2BP1, IGF2BP3, BMI1, and γ-globin protein in control (sgNeg: nontargeting sgRNA; sgBCL11A+58, positive control) or BMI1-depleted primary adult erythroblasts. GAPDH was used as loading control. DEGs, differentially expressed genes.

IGF2BP1 and IGF2BP3 mediate the HbF silencing activity of BMI1 in primary adult erythroblasts. (A) Schematic of BMI1 depletion experiments in primary adult erythroid cells. (B-C) Volcano plots of DEGs identified from RNA-seq in BMI1-depleted primary adult erythroblasts (sgBMI1#1 or sgBMI1#3) by DESeq2. Cutoff threshold: fold change > 2; FDR < 0.1; n = 2. (D) Venn diagram of overlapped DEGs identified from RNA-seq in BMI1-depleted primary adult erythroblasts by comparing control sample with sgBMI1#1 (B) and sgBMI1#3 (C) sample, respectively. (E) Representative immunoblots of BCL11A, IGF2BP1, IGF2BP3, BMI1, and γ-globin protein in control (sgNeg: nontargeting sgRNA; sgBCL11A+58, positive control) or BMI1-depleted primary adult erythroblasts. GAPDH was used as loading control. DEGs, differentially expressed genes.

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