Single-cell transcriptome analysis. (A) t-distributed stochastic neighbor embedding (tSNE) plot of the integrated WMCL1 analysis. The colors highlight the different cell clusters; the numbers reported at the top of each cluster indicate the associated cluster index. (B) tSNE plots highlighting the expression level of B-cell (top) and Tregs (bottom) markers in a yellow (high expression) to blue (low expression) color scale. (C) tSNE plot representing the different cell types at cluster resolution. (D) Percentage of Tregs after exposure to normal CD19 cells, to WMCL1, or to WMCL1-CXCR4^C1013G cells.