Tregs obtained from patients with WM present with a peculiar transcriptome signature that differs from HD-Tregs. (A,B) Bulk RNA-seq comparing WM-Tregs (n = 14) and HD-Tregs (n = 8). WM-Tregs present with a significant enrichment of FOXP3, and Treg-, TNF-α–, interferon-related genes, as assessed by GSEA. Normalized enrichment score (NES) was generated by comparing WM-Tregs and HD-Tregs. NES, nominal P value, and false discovery rate q values are shown for each plot. (C) Network plot showing the top leading-edge genes of the GSEA signatures associated with Treg activity. Genes are reported in yellow and GSEA signatures in light blue.