The expression of p120-catenin results in a striking difference in the signal transduction of TIM-3 between AML and exhausted T cells. (A) WB analysis of Gal-9–stimulated TIM-3-OX THP-1 and PD-1⁺TIM-3⁺ exhausted T cells. Of note, Gal-9 ligation to TIM-3 induced LRP6 phosphorylation in TIM-3-OX THP-1 cells but not in the exhausted T cells devoid of p120-catenin expression. (B) RT-qPCR analysis of CTNND1 mRNA in FACS-purified AML cells and the exhausted (PD-1⁺TIM-3⁺) T cells from 2 patients with AML (AML#12 and #15). Results were normalized to the mRNA expression of GAPDH. Data are presented as mean ± SEM, ∗P < .05. (C) Intracellular FACS analysis of p120-catenin expression in TIM-3⁺ AML cells and PD-1⁺TIM-3⁺ exhausted T cells in the identical patient with AML. A representative result out of 3 AML samples (AML#8, #12, and #15) is shown. (D) FACS analysis of surface TIM-3 expression in Ki-JK cells. TIM-3 expression of KASUMI-3 and KASUMI-6 are shown as positive controls. (E) The results of WB analysis using mock-transfected (left panels) and p120-catenin-OX Ki-JK cells (right panels) stimulated with Gal-9 are shown.