Figure 1.
![Overview of the role of genome/gene-editing platforms. Genome-editing nucleases (TALEN, ZFN, and CRISPR-Cas9) induce DSBs within the nuclear DNA at specific sites, which can subsequently be repaired using nonhomologous end-joining (NHEJ) or homology-directed repair (HDR) pathways that assist in targeted modification of the DNA. In contrast, gene-editing RNA interference strategies (using shRNA or small interfering RNA [siRNA]) bind to complementary messenger RNA sequences within the cytoplasm and cause its degradation. mRNA, messenger RNA; RISC, RNA-induced silencing complex. Created with www.BioRender.com.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/7/10/10.1182_bloodadvances.2022008966/1/blooda_adv-2022-008966-gr1.jpeg?Expires=1724949888&Signature=enrFGCV3nJPHLYK-HOLg-FuUCTqbTNdMjm3bJYxlD-oK14ulo9IqSKND1McGdO9V6K9dlesGhiztAPf7hYqn29g9KMg07zHKp5jkH3yoLhdzmnsfI~RLpO22WcfTx4xIPYIsCIdla4O~BNyeMsdo61jeDUiHLgFfy4TlmqR0s9BTnPU6tDDspTWi3upkCOFWEaOGpEkn75VO2L3BSPklZJ19-JHDv7RWkXJF62CDF4tiE5wobi9IW0LCxinR4RtvqU-Hc7Nsi4Rg9hYveLYqmT2q~jIlXTOlhZ7Bwp83D1XV5XYonZunW56oPemHPsjCpw2Kedf76qzNY3llLiCNJA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Overview of the role of genome/gene-editing platforms. Genome-editing nucleases (TALEN, ZFN, and CRISPR-Cas9) induce DSBs within the nuclear DNA at specific sites, which can subsequently be repaired using nonhomologous end-joining (NHEJ) or homology-directed repair (HDR) pathways that assist in targeted modification of the DNA. In contrast, gene-editing RNA interference strategies (using shRNA or small interfering RNA [siRNA]) bind to complementary messenger RNA sequences within the cytoplasm and cause its degradation. mRNA, messenger RNA; RISC, RNA-induced silencing complex. Created with www.BioRender.com.
