Transcriptomic heterogeneity of human AITL samples analyzed at single-cell resolution. (A) Overview of scRNA-seq for AITL (n = 5) and homeostatic lymph nodes (HLNs, n = 3). (B) List of somatic mutations detected using WES in AITL tissues. (C) UMAP plot after integrating the scRNA-seq data of AITL and HLN. Three lineage clusters (T cells [T]; B cells [B]; and myeloid cells [MYE]) and a cluster characterized by proliferative markers (PRO) are indicated by dashed circles. (D) Pie graphs showing the proportions of each cluster in the AITL and HLN. Numbers in parentheses indicate the percentages of each cluster. (E) UMAP plot of T-cell subclusters sorted in silico after integrating scRNA-seq data of AITL and HLN samples. Six subclusters were labeled with different colors. (F) Bar graphs showing the percentages of each T-cell subcluster in the AITL or HLN samples. (G) Heatmap of the top 50 conserved markers in each T-cell subcluster from (E). (H) Stacked violin plots showing specific conserved markers expressed in each cluster. (I) Feature plots showing CD4, CD8A, and specific markers for each T-cell subcluster. (J) UMAP plot depicting TCR clone size for each clonotype.