AUTO3 study design and recruitment. (A) AUTO3 CAR, CD19 CAR, and CD22 CAR are type I transmembrane proteins. Human HD37 recognizes CD19, is located at the extreme amino terminus and, in turn, connected to the CD8α stalk, transmembrane, and anchor. The intracellular endodomains are composed of costimulatory OX40 and CD3 ζ (TCRζ). Humanized LT22 recognizes CD22, is located at the extreme amino terminus and is connected to the pentameric α-helical coiled coil multimer forming domain of cartilage oligomeric matrix protein (COMP) and transmembrane anchor. The intracellular endodomains are composed from 41BB ζ and CD3 ζ (TCRζ).¹⁴ (B) AUTO3 trial schema. (C) AUTO3 consort diagram. Cy, cyclophosphamide; Flu, fludarabine; NT, nontransduced; scFV, single chain variable fragment; TCRz, T-cell receptor zeta.