NBSGW mice showed superior human HSPC engraftment compared with NSG mice. A total of 1 × 106 human CD34+ cells isolated from G-CSF mobilized donors were IV-transplanted to 8- to 10-week-old NSG (n = 16) and NBSGW (n = 9) mice. (A) Percentages of human CD45+ cells in total PBMCs were measured by flow cytometry to determine engraftment efficiency at 10 weeks after transplant. (B) Transplanted NSG (n = 6) and NBSGW (n = 9) mice were intraperitoneally injected 3 times with Clodronate liposomes (CL; 100 ul per injection) over a week. Human CD41+ platelet concentrations were evaluated by flow cytometry. The data are presented as means with standard deviation. ∗∗P < .01 and ∗∗∗P < .001. (C) Representative flow panels to show the proportion of human platelets and mouse platelets in NBSGW mice 1 day following CL treatment. Left, before CL treatment; right, after CL treatment.
