Figure 3.
Endogenous MOZ is required for active histone modifications at the Meis1 locus.Moz+/+, Moz+/−, or Moz−/−MOZ-TIF2 and MLL-AF9 AML cells were fixed with formalin, and ChIP-Seq or ChIP-qPCR assays were performed. (A) ChIP-Seq analysis of Histone H3 K9 and K23 acetylation at the Hoxa cluster and Meis1 loci in Moz+/+ and Moz−/− MOZ-TIF2 AML cells. (B) Active histone modifications at the Meis1, Hoxa9 loci, and a mouse chromosome 3 desert (Chr. 3 desert) (negative control) loci in MOZ-TIF2 AML cells. Histone modifications at Meis1, Hoxa9 loci, and a Chr. 3 desert loci were measured by quantitative PCR analysis. Representative results of ChIP assays are shown. Seven independent experiments were conducted in MOZ-TIF2 AML cells. Primer sets used to amplify the Meis1 and Hoxa9 loci are indicated at the bottom. Levels of each histone modification were normalized to input DNA and total histone H3 concentrations. Error bars represent mean ± SD.

Endogenous MOZ is required for active histone modifications at the Meis1 locus.Moz+/+, Moz+/−, or Moz−/−MOZ-TIF2 and MLL-AF9 AML cells were fixed with formalin, and ChIP-Seq or ChIP-qPCR assays were performed. (A) ChIP-Seq analysis of Histone H3 K9 and K23 acetylation at the Hoxa cluster and Meis1 loci in Moz+/+ and Moz−/− MOZ-TIF2 AML cells. (B) Active histone modifications at the Meis1, Hoxa9 loci, and a mouse chromosome 3 desert (Chr. 3 desert) (negative control) loci in MOZ-TIF2 AML cells. Histone modifications at Meis1, Hoxa9 loci, and a Chr. 3 desert loci were measured by quantitative PCR analysis. Representative results of ChIP assays are shown. Seven independent experiments were conducted in MOZ-TIF2 AML cells. Primer sets used to amplify the Meis1 and Hoxa9 loci are indicated at the bottom. Levels of each histone modification were normalized to input DNA and total histone H3 concentrations. Error bars represent mean ± SD.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal