![Endogenous MOZ is essential for MOZ/MLL fusion-induced leukemia development. (A) Serial colony replating assays were performed in Moz+/+, Moz+/−, and Moz−/− HSPCs bearing MOZ-TIF2, MLL-AF9, MLL-AF10, or HOXA9. A scheme of the experimental workflow is shown at the top. First, Moz+/+, Moz+/−, or Moz−/− FL lineage− HSPCs (2 × 105 cells) were transduced with MOZ-TIF2, MLL-AF10, MLL-AF9, or HOXA9 and cultured in a liquid medium. Subsequently, GFP+ cells (5 × 104) were sorted and cultured in a methylcellulose medium. Every 4 to 5 days, colony and cell numbers (× 105 cells) were counted, and then 1 × 105 cells were serially replated 5 times. Mean values were calculated from 3 to 4 independent experiments. Error bars represent mean ± standard deviation (SD). (B) Overall survival of recipient mice transplanted with Moz+/+, Moz+/−, or Moz−/− HSPCs (2 × 105) bearing MOZ-TIF2, MLL-AF10, or HOXA9 and MEIS1 (MOZ-TIF2, Moz+/+ and Moz−/− [n = 6], Moz+/− [n = 12]; MLL-AF10, Moz+/+ [n = 6], Moz+/− [n = 10], Moz−/− [n = 4]; HOXA9 + MEIS1, [n = 6]). Differences in survival were compared using the log-rank test; ∗P < .05, ∗∗P < .01, and ∗∗∗P < .005.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/6/19/10.1182_bloodadvances.2020003490/11/blooda_adv-2020-003490-gr1.jpeg?Expires=1724236378&Signature=EXdd2-RHiHpqayO8l3Hwv9Cfailg88SNhWDjQM4-dRr5unkh8L7yYULrih4Ek0l06xwuXpMg46MOIBJEI3nCm9Sv9lVi~duwM4HcwRpqKxBy~t2-1~2DyUCH7xqJZECmESvCzMoBUmu-Ipgzt2lTv-wIdK~-1RhzpMyxarrX7M5B8J6cBuSv3s7rD2mgS8GOXCNm~AjMOsgSqwuaHXumruQbUrtzoYRigrKa-spGH23m3-TwuhYlySjSPkaJPEKVOAJ~Ns2BJfpzKxk9TsSp6l4Bhg1bNBlN0UItF-GKNmMsrv08By2hh3Z2XSbRtBHDnN-BD7LyTjhr1g0A3wn~IQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Endogenous MOZ is essential for MOZ/MLL fusion-induced leukemia development. (A) Serial colony replating assays were performed in Moz+/+, Moz+/−, and Moz−/− HSPCs bearing MOZ-TIF2, MLL-AF9, MLL-AF10, or HOXA9. A scheme of the experimental workflow is shown at the top. First, Moz+/+, Moz+/−, or Moz−/− FL lineage− HSPCs (2 × 105 cells) were transduced with MOZ-TIF2, MLL-AF10, MLL-AF9, or HOXA9 and cultured in a liquid medium. Subsequently, GFP+ cells (5 × 104) were sorted and cultured in a methylcellulose medium. Every 4 to 5 days, colony and cell numbers (× 105 cells) were counted, and then 1 × 105 cells were serially replated 5 times. Mean values were calculated from 3 to 4 independent experiments. Error bars represent mean ± standard deviation (SD). (B) Overall survival of recipient mice transplanted with Moz+/+, Moz+/−, or Moz−/− HSPCs (2 × 105) bearing MOZ-TIF2, MLL-AF10, or HOXA9 and MEIS1 (MOZ-TIF2, Moz+/+ and Moz−/− [n = 6], Moz+/− [n = 12]; MLL-AF10, Moz+/+ [n = 6], Moz+/− [n = 10], Moz−/− [n = 4]; HOXA9 + MEIS1, [n = 6]). Differences in survival were compared using the log-rank test; ∗P < .05, ∗∗P < .01, and ∗∗∗P < .005.
