Figure 4.
OTKs-positive cells stimulate DNA resection and TMEJ/MMEJ. (A-B) BaF3 and 32D parental cells and the cells expressing JAK2(V617F), FLT3(ITD), or BCR-ABL1 were mock-treated or irradiated (10 Gy). (A) Immunofluorescence against BrdU and PCNA is shown. (B) The data show the mean ± SD; ∗P ≤ .05, ∗∗P ≤ .0001 using Mann-Whitney U test. (C) DPC-TMEJ assay and (D) pBABE-MMEJ assay. DPC-TMEJ or pBABE-MMEJ reporters and DsRed plasmid were transiently transfected in to parental 32Dcl3 cells (P) and cells expressing FLT3(ITD), JAK2(V617F), and BCR-ABL1 followed by flow cytometry. Results represent the mean percentage of GFP+ cells in DsRed+ population ± SD. ∗P < .05 when compared with P using t test. BrdU, 5-bromo-2′-deoxyuridine; SD, standard deviation.

OTKs-positive cells stimulate DNA resection and TMEJ/MMEJ. (A-B) BaF3 and 32D parental cells and the cells expressing JAK2(V617F), FLT3(ITD), or BCR-ABL1 were mock-treated or irradiated (10 Gy). (A) Immunofluorescence against BrdU and PCNA is shown. (B) The data show the mean ± SD; ∗P ≤ .05, ∗∗P ≤ .0001 using Mann-Whitney U test. (C) DPC-TMEJ assay and (D) pBABE-MMEJ assay. DPC-TMEJ or pBABE-MMEJ reporters and DsRed plasmid were transiently transfected in to parental 32Dcl3 cells (P) and cells expressing FLT3(ITD), JAK2(V617F), and BCR-ABL1 followed by flow cytometry. Results represent the mean percentage of GFP+ cells in DsRed+ population ± SD. ∗P < .05 when compared with P using t test. BrdU, 5-bromo-2′-deoxyuridine; SD, standard deviation.

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