Distribution of AAV ISs within Tcr loci. (A-C) Genomic distribution of AAV IS in the LN and SPL data sets of ZAP70-treated mice and targeting TCR genes: TCRα (A), TCRβ (B), and TCRγ (C), as indicated. Genomic coordinates and scale are indicated in each panel. Black and blue lines indicate the position of the AAV IS from the LN (black) and SPL (blue) data sets. V, D, and J segments are indicated by yellow, blue, and purple rectangles, respectively. (C) Constant regions are represented as brown rectangles. Clusters of AAV ISs are identified in TCR genes, especially in the J-segment region, whose genomic area is enlarged below the Tcrα and Tcrβ loci; gene segment genomic coordinates were retrieved from IMGT, the international ImMunoGeneTics information system. (D) Gene targeting frequency (indicated as percentage) of Tcrα, Tcrβ, and Tcrγ in the different AAV ISs data sets as indicated.